Bioinformatics Prediction of Polyketide Synthase Gene Clusters from Mycosphaerella fijiensis
نویسندگان
چکیده
منابع مشابه
Bioinformatics Prediction of Polyketide Synthase Gene Clusters from Mycosphaerella fijiensis
Mycosphaerella fijiensis, causal agent of black Sigatoka disease of banana, is a Dothideomycete fungus closely related to fungi that produce polyketides important for plant pathogenicity. We utilized the M. fijiensis genome sequence to predict PKS genes and their gene clusters and make bioinformatics predictions about the types of compounds produced by these clusters. Eight PKS gene clusters we...
متن کاملIsolation of retro-transcribed RNA from in vitro Mycosphaerella fijiensis-infected banana leaves.
High polyphenol and polysaccharide levels in plant tissues such as banana fruit and leaves constitute a significant challenge to the extraction of sufficient amounts of high-quality RNA required for cDNA library synthesis and molecular analysis. To determine their comparative effectiveness at eliminating polyphenols, polysaccharides and proteins, three protocols for RNA extraction from in vitro...
متن کاملAnalysis of Expressed Sequence Tags from the Fungal Banana Pathogen Mycosphaerella fijiensis
Mycosphaerella fijiensis is a fungal pathogen causing black leaf streak disease in banana plants. In order to develop our understanding of the molecular mechanisms of host infection by M. fijiensis, we initiated a first step towards large-scale gene discovery through the generation and analysis of expressed sequence tags (ESTs). Three cDNA libraries representing different culture conditions wer...
متن کاملStructural and functional characterization of three polyketide synthase gene clusters in Bacillus amyloliquefaciens FZB 42.
Although bacterial polyketides are of considerable biomedical interest, the molecular biology of polyketide biosynthesis in Bacillus spp., one of the richest bacterial sources of bioactive natural products, remains largely unexplored. Here we assign for the first time complete polyketide synthase (PKS) gene clusters to Bacillus antibiotics. Three giant modular PKS systems of the trans-acyltrans...
متن کاملChemical management in fungicide sensivity of Mycosphaerella fijiensis collected from banana fields in México
The chemical management of the black leaf streak disease in banana caused by Mycosphaerella fijiensis (Morelet) requires numerous applications of fungicides per year. However this has led to fungicide resistance in the field. The present study evaluated the activities of six fungicides against the mycelial growth by determination of EC50 values of strains collected from fields with different fu...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: PLOS ONE
سال: 2016
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0158471